Water On-Line Analysis (WOLA) leads cutting-edge analytical technology to analyze biological parameters of water quality quickly and effectively, decreasing analysis time from 5 to 30 minutes and cost for analysis compared with other traditional techniques. The equipments developed may help to control, improve and optimize treatment and industrial processes of WWTP, green industries and fish farms. Also, public bodies may be supported taking real-time control of toxic substances in natural resources and environment. Important national and international organisms (PROMOTE-ETV, BIOSET) have awarded the great advances of WOLA?s technology.